Dr. rer. nat. Vladimir Zverlov

Room No. 024

Tel.: +49 8161 / 71 5474

E-Mail: vladimir.zverlov[at]tum.de

 

Vladimir received his Ph.D. in molecular genetics in 1994 and he has over 30 years of experience in Clostridium thermocellum research. His results were published in >90 peer reviewed journals and he was several time awarded. In 2007 he received the Liebig-Medaille (Süd-Chemie AG) for his contribution in the area of bio-catalysis. In 2001-2002 he was awarded by the Alexander von Humboldt foundation and received several DFG awards.

 

curriculum vitae

since 2005 Technical University Munich, Institute for Microbiology (Prof. Liebl), Research Group for Microbial Biotechnology, Working Group Leader and Head of Laboratory
1996-2005 Russian Academy of Sciences, Moscow, Russia, Institute of Molecular Genetics, Laboratory of Molecular Genetics of Anaerobic Bacteria, Senior Scientist
1986-1996 Russian Academy of Sciences, Moscow, Russia, Institute of Molecular Genetics, Laboratory of Molecular Genetics of Anaerobic Bacteria, Scientist
1994 Russian Academy of Sciences, Moscow, Russia, Institute of Molecular Genetics, Laboratory of Molecular Genetics of Anaerobic Bacteria, Doctoral thesis on "The study of structure of the Clostridium thermocellum F7 lichenase gene and characterization of the gene product"
1986 Institute of Physics and Technology, Moscow, Russia (State University), Diploma thesis

 

Published articles:

100. Rettenmaier, R., Gerbaulet, M., Liebl, W., Zverlov, V.V. (2019) Hungateiclostridium mesophilum sp. nov., a mesophilic, cellulolytic and spore-forming bacterium isolated from a biogas fermenter fed with maize silage. International Journal of Systematic and Evolutionary Microbiology.

99. Rykov, S.V., Kornberger, P., Herlet, J., Berezina, O.V.,  Tsurin, N.V., Zorov, I.N., Zverlov V.V., Liebl, W., Schwarz, W.H., Yarotsky S.V., Berezina O. (2019) Novel endo-(1,4)-ß-glucanase Bgh12A and xyloglucanase Xgh12B from Aspergillus cervinus belong to GH12 subgroup I and II, respectively. Applied Microbiology and Biotechnology. doi.org/10.1007/s00253-019-10006-x

98. Rettenmaier, R., Duerr, C., Neuhaus, K., Liebl, W., Zverlov, V.V. (2019) Comparison of sampling techniques and different media for the enrichment and isolation of cellulolytic organisms from biogas fermenters. Systematic and Applied Microbiology 42:481-487. doi.org/10.1016/j.syapm.2019.05.002

97. Rettenmaier, R., Neuhaus, K., Liebl, W., Zverlov, V.V. (2019) Draft genome sequence of Anaerosphaera sp. strain GS7-6-2, a coccal bacterium isolated from a biogas-related environment. Microbiol Resour Announc. 8:e00205-19. doi.org/10.1128/MRA.00205-19

96. Heinze, S., Zimmermann, K., Ludwig, C., Heinzlmeir, S., Schwarz, W.H., Zverlov, V.V., Liebl, W., Kornberger, P. (2018) Evaluation of promoter sequences for the secretory production of a Clostridium thermocellum cellulase in Paenibacillus polymyxa. Applied Microbiology and Biotechnology 102:10147-10159. doi.org/10.1007/s00253-018-9369-7

95. Herlet, J., Schwarz, W.H., Zverlov, V. V., Liebl, W., Kornberger, P. (2018) Addition of ß-galactosidase boosts the xyloglucan degradation capability of endoglucanase Cel9D from Clostridium thermocellum. Biotechnology for Biofuels 11:238. doi.org/10.1186/s13068-018-1242-5

94. Broeker, J., Mechelke, M., Baudrexl, M., Mennerich, D., Hornburg, D., Mann, M., Schwarz, W.H., Liebl, W., Zverlov, V.V. (2018) The hemicellulose-degrading enzyme system of the thermophilic bacterium Clostridium stercorarium: comparative characterisation and addition of new hemicellulolytic glycoside hydrolases. Biotechnology for Biofuels 11:229. doi.org/10.1186/s13068-018-1228-3

93. Leis, B., Held, C., Andreeßen, B., Liebl, W., Graubner, S., Schulte, L.P.,  Schwarz, W.H., Zverlov, V.V. (2018) Optimizing the composition of a synthetic cellulosome complex for the hydrolysis of softwood pulp - identification of the enzymatic core functions and biochemical complex characterization. Biotechnology for Biofuel 11:220. doi.org/10.1186/s13068-018-1220-y

92. Heinze, S., Kornberger, P., Graetz, C., Schwarz, W.H., Zverlov, V.V., Liebl, W. (2018) Conjugative transfer of a new broad host range expression vector to various Bacillus species using a single protocol. BMC Microbiology 18:56. doi.org/10.1186/s12866-018-1198-4

91. Schwarz, W.H., Brunecky, R., Broeker, J., Liebl, W., Zverlov, V.V. (2018) Handling gene and protein names in the age of bioinformatics - the special challenge of secreted multimodular bacterial enzymes such as the cbhA/cbh9A gene of Clostridium thermocellum. World Journal of Microbiology and Biotechnology 34:42. doi.org/10.1007/s11274-018-2424-9

90. Pechtl, A., Rückert, C., Maus, I., Koeck, D.E., Trushina, N., Kornberger, P., Schwarz, W.H., Schlüter, A., Liebl, W., Zverlov, V. V. (2018) Complete Genome Sequence of the Novel Cellulolytic, Anaerobic, Thermophilic Bacterium Herbivorax saccincola Type Strain GGR1, Isolated from a Lab Scale Biogas Reactor as Established by Illumina and Nanopore MinION Sequencing. Genome Announc. 6:e01493-17. doi.org/10.1128/genomeA.01493-17

89. Dvortsov, I.A., Lunina, N.A., Chekanovshaya, L.A., Gromov, A.V., Schwarz, W.H., Zverlov, V. V., Velikodvorskaya, G. A., Demidyuk, I.V., Kostrov, S. V. (2018) Carbohydrate Binding Module CBM28 of Endoglucanase Cel5D from Caldicellulosiruptor bescii Recognizes Crystalline Cellulose. International Journal of Biological Macromolecules. 107:305-311. doi.org/10.1016/j.ijbiomac.2017.08.165.

88. Leis, B., Held, C., Bergkemper, F., Dennemarck, K., Steinbauer, R., Reiter, A., Mechelke, M., Moerch, M., Graubner, S., Liebl, W., Schwarz, W.H., Zverlov, V.V. (2017) Comparative characterization of all cellulosomal cellulases from Clostridium thermocellum reveals high diversity in endoglucanase product formation essential for complex activity. Biotechnology for Biofuels 10:240.  doi.org/10.1186/s13068-017-0928-4

87. Herlet, J., Kornberger, P., Roessler, B., Glanz, J., Schwarz, W.H., Liebl, W., Zverlov, V. V. (2017) A new method to evaluate temperature vs. pH activity profiles for biotechnological relevant enzymes. Biotechnology for Biofuels 10:234. doi.org/10.1186/s13068-017-0923-9

86. Mechelke, M., Herlet, J., Benz, J.P., Schwarz, W.H., Zverlov, V. V., Liebl, W., Kornberger, P. (2017) HPAEC-PAD for oligosaccharide analysis – Novel insights into analyte sensitivity and response stability. Analytical and Bioanalytical Chemistry 409:7169-7181. doi.org/10.1007/s00216-017-0678-y.

85.  Maus, I., Bremges, A., Stolze, Y., Hahnke, S., Cibis, K.G., Koeck, D.E., Kim, Y.S., Kreubel, J., Hassa, J., Wibberg, D., Weimann, A., Off, S., Robbin Stantscheff; Zverlov, V.V.,  Schwarz, W.H., König, H., Liebl, W., Scherer, P., McHardy, A.C., Sczyrba, A., Klocke, M., Pühler, A., Schlueter, A. (2017) Genomics and prevalence of bacterial and archaeal isolates from biogas-producing microbiomes. Biotechnology for Biofuels 10:264. doi.org/10.1186/s13068-017-0947-1

84. Heinze, S.,Mechelke, M., Kornberger, P., Liebl, W., Schwarz, W.H., Zverlov, V.V. (2017) Identification of endoxylanase XynE from Clostridium thermocellum as the first xylanase of glycoside hydrolase family GH141. Scientific Reports 7(1):11178. doi.org/10.1038/s41598-017-11598-y.

83. Mechelke, M., Koeck, D.E., Broeker, J.K., Roessler, B., Krabichler, F., Schwarz, W.H., Zverlov, V.V., Liebl, W. (2017) Characterization of the arabinoxylan-degrading machinery of the thermophilic bacterium Herbinix hemicellulosilytica - six new xylanases, three arabinofuranosidases and one xylosidase. J. Biotechnol. 257:122-130. doi.org/10.1016/j.jbiotec.2017.04.023.

82. Berezina, O.V., Herlet, J., Rykov, S.V., Kornberger, P., Zavyalov, A., Kozlov, D., Sakhibgaraeva, L.F., Krestyanova, I.N., Schwarz, W.H., Zverlov, V.V., Liebl, W., Yarotsky, S.V. (2017) Thermostable multifunctional GH74 xyloglucanase from Myceliophthora thermophila: High-level expression in Pichia pastoris and characterization of the recombinant protein. Applied Microbiology and Biotechnology 101:5653–5666. doi.org/10.1007/s00253-017-8297-2

81. Krestyanova, I.N., Sakhibgaraeva, L.F., Berezina, O.V., Rykov, S.V., Zavyalov, A.V., Zverlov, V.V., Yarotsky, S.V. (2016) Characteristics of Fungal Strains Producing the Thermostable Xyloglucanases from the Russian National Collection of Industrial Microorganisms. Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya (Molecular Genetics, Microbiology and Virology) 34(3): 4–11 (Russian). doi.org/10.18821/0208-0613-2016-34-3-109-114.

80. Koeck, D.E., Mechelke, M., Zverlov, V.V., Liebl, W., Schwarz, W.H. (2016) Herbivorax saccincola gen. nov., sp. nov., a cellulolytic, anaerobic, thermophilic bacterium isolated via in sacco enrichments from a lab scale biogas reactor. International Journal of Systematic and Evolutionary Microbiolog. 66: 4458-4463. doi.org/10.1099/ijsem.0.001374

79. Maus, I. , Koeck, D.E., Cibis, K.G., Hahnke, S., Kim, Y.S., Langer, T., Kreubel, J., Erhard, M., Bremges,  A., Off, S., Stolze, Y., Jaenicke, S., Goesmann, A., Sczyrba, A., Scherer, P., König, H., Schwarz, W.H., Zverlov, V.V., Liebl, W., Pühler, A., Schlüter, A., Klocke, M. (2016). Unraveling the microbiome of a thermophilic biogas plant by metagenome and metatranscriptome analysis complemented by characterization of bacterial and archaeal isolates. Biotechnology for Biofuels. 9: 171.  doi.org/10.1186/s13068-016-0581-3

78. Koeck, D.E., Hahnke, S., Zverlov, V.V. (2016). Herbinix luporum sp. nov., a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor. International Journal of Systematic and Evolutionary Microbiology 66, 4132-4137. doi.org/10.1099/ijsem.0.001324

77. Koeck, D.E, Maus, I., Wibberg, D., Winkler, A., Zverlov, V.V.,  Liebl, W., Pühler, A., Schwarz, W.H., Schlüter A. (2016). Complete genome sequence of Herbinix luporum SD1D, a new cellulose degrading bacterium isolated from a thermophilic biogas reactor.  Genome Announc 4(4), e00687-16.  doi.org/10.1128/genomeA.00687-16

76. Koeck, D.E, Maus, I., Wibberg, D., Winkler, A., Zverlov, V.V.,  Liebl, W., Pühler, A., Schwarz, W.H., Schlüter A. (2016). Draft genome sequence of Propionispora sp. 2/2-37, a new xylan degrading bacterium isolated from a mesophilic biogas reactor. Genome Announc 4(3), e00609-16. doi.org/10.1128/genomeA.00609-16

75. Koeck, D.E., Wibberg, D., Winkler, A., Zverlov, V.V., Liebl, W., Pühler, A., Schwarz, W.H., Schlüter A. (2015). Draft genome sequence of Herbinix hemicellulosilytica T3/55T, a new thermophilic cellulose degrading bacterium isolated from a thermophilic biogas reactor. J. Biotechnol. 214: 59-60.  doi.org/10.1016/j.jbiotec.2015.07.022

74. Koeck, D. E., Koellmeier, T., Schiffner, S., Zverlov, V. V., Liebl, W., Schwarz, W. H. (2015). Differences in biomass degradation between newly isolated environmental strains of Clostridium thermocellum and heterogeneity in the size of the cellulosomal scaffoldin. Systematic and Applied Microbiology 38: 424-432. doi.org/10.1016/j.syapm.2015.06.005

73.Koeck, D. E., Ludwig, W., Wanner, G., Zverlov, V. V., Liebl, W., Schwarz, W.H. (2015). Herbinix hemicellulosilytica, gen. nov., sp. nov., a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor. International Journal of Systematic and Evolutionary Microbiology 65: 2365-2371. doi.org/10.1099/ijs.0.000264

72. Zverlov, V. V., Koeck, D. E., Schwarz, W. H. (2015). The Role of Cellulose-Hydrolyzing Bacteria in the Production of Biogas from Plant Biomass. In B. Kamm (ed.), Microorganisms in Biorefineries, Microbiology Monographs 26: 335-361. doi.org/10.1007/978-3-662-45209-7_12

71. Dominguez, O.V., Peters, W., Reinbothe, C., Schwarz, W., Zverlov, V., Beck, E. (2014) Characterization of a Glycan Exo-Hydrolase that Shows a Biphasic Expression in the Course of an In Vitro Culture of Photoautotrophic Oxybasis rubra Cells. J Plant Biochem Physiol 2: 137. doi.org/10.4172/2329-9029.1000137

70. Koeck, D. E., Wibberg, D., Maus, I., Winkler, A., Albersmeier, A., Zverlov, V. V., Pühler, A., Schwarz, W. H., Liebl, W., Schlüter, A. (2014). First draft genome sequence of the amylolytic Bacillus thermoamylovorans wild-type strain 1A1 isolated from a thermophilic biogas plant. J. Biotechnol. 192A: 154-155. doi.org/10.1016/j.jbiotec.2014.09.017

69. Koeck, D. E., Wibberg, D., Maus, I., Winkler, A., Albersmeier, A., Zverlov, V. V., Liebl, W., Pühler, A., Schwarz, W. H., Schlüter, A. (2014). Complete genome sequence of the cellulolytic thermophile Ruminiclostridium cellulosi wild-type strain DG5 isolated from a thermophilic biogas plant. J. Biotechnol. 188C:136-137. doi.org/10.1016/j.jbiotec.2014.08.024

68. Koeck, D. E., Pechtl, A., Zverlov, V. V., Schwarz, W. H. (2014). Genomics of cellulolytic bacteria. Current Opin. Biotechnol. 29:171-183. doi.org/10.1016/j.copbio.2014.07.002

67. Köck, D.E., Zverlov, V. V., Liebl, W., Schwarz, W. H. (2014). Comparative genotyping of Clostridium thermocellum strains isolated from biogas plants: genetic markers and characterization of cellulolytic potential. Systematic and Applied Microbiology 37(5), 311-319. doi.org/10.1016/j.syapm.2014.05.006

66. Panitz, J.C., Zverlov, V. V., Pham, V.T.T., Stürzl, S., Schieder, D., Schwarz, W. H. (2014). Isolation of a solventogenic Clostridium sp. strain: Fermentation of glycerol to n-butanol, analysis of the bcs operon region and its potential regulatory elements. Systematic and Applied Microbiology 37, 1-9. doi.org/10.1016/j.syapm.2013.10.004

65. Köck, D.E., Wibberg, D., Köllmeier, T., Blom, J., Jaenicke, S., Winkler, A., Albersmeier, A., Zverlov, V. V., Pühler, A., Schwarz, W. H., Schlüter, A. (2013). Draft genome sequence of the cellulolytic Clostridium thermocellum wild-type strain BC1 playing a role in cellulosic biomass degradation. Journal of Biotechnology 168, 62-63. https://doi.org/10.1016/j.jbiotec.2013.08.011

64. Velikodvorskaya, G. A., Chekanovshaya, L.A., Lunina, N.A., Sergienko, O.V., Lunin, V. V., Dvortsov, I.A., Zverlov, V. V. (2013) Family 28 carbohydrate-binding module of the thermostable endo-1,4-ß-glucanase CelD from Caldicellulosiruptor bescii maximizes enzyme activity and irreversibly binds to amorphous cellulose. Molecular Biology. 47(4), 581-586. doi.org/10.1134/S0026893313040158

63. Poehlein, A., Zverlov, V. V., Daniel, R., Schwarz, W. H., Liebl, W. (2013). Complete genome sequence of Clostridium stercorarium subsp. stercorarium strain DSM 8532, a thermophilic degrader of plant cell wall fibers. Genome Announc. 1(2), e00073-13. doi.org/10.1128/genomeA.00073-13

62. Köllmeier, T., Zverlov, V. V., Schwarz, W. H. (2012). Microbiology of plant fibre hydrolysis in biogas plants. Bornimer Agrartechnische Berichte 79, ISSN 0947-7314, 99-113. 

61. Dvortsov, I. A., Lunina, N. A, Zverlov, V. V. and Velikodvorskaya, G. A. (2012). The properties of four C-therminal carbohydrate-binding modules (CBM4) of laminarinase Lic16A of Clostridium thermocellum. Molecular Biology 46, 819-824. doi.org/10.1134/S0026893312060039

60. Kraus, J., Zverlov, V. V. and Schwarz, W. H. (2012). In Vitro Reconstitution of the Complete Clostridium thermocellum Cellulosome and Synergistic Activity on Crystalline Cellulose. Applied and Enviromental Microbiology 78(12), 4301-4307. doi.org/10.1128/AEM.07959-11

59. Berezina, O.V., Zakharova, N. V., Yarotsky, S. V. , Zverlov, V. V. (2011). Microbial producers of butanol. Biotechnology 4 (Russ.), 8-25.2012. doi.org/10.1134/S0003683812070022

58. Zverlov, V. V., Hiegl, W., Köck, D. E., Kellermann, J., Köllmeier, T., Schwarz, W. H. (2010). Hydrolytic bacteria in mesophilic and thermophilic degradation of plant biomass. Engineering in Life Sciences 10, 528-536. doi.org/10.1002/elsc.201000059

57. Dvortsov, I. A., . Lunina, N. A, Zverlov, V. V. and Velikodvorskaya, G. A. (2010). Substrate Binding Properties of the Family 54 Module of Clostridium thermocellum Lic16A Laminarinase. Molecular Biology 44, 591-595. doi.org/10.1134/S002689331004014X

56. Berezina, O.V., Zakharova, N. V., Brandt, A., Yarotsky, S. V. , Schwarz, W. H., Zverlov, V. V. (2010). Reconstructing the clostridial n-butanol metabolic pathway in Lactobacillus brevis. Applied Microbiology and Biotechnology 87, 635-646. doi.org/10.1007/s00253-010-2480-z

55. Berezina, O.V., Brandt, A., Yarotsky, S., Schwarz, W. H., Zverlov, V. V. (2009). Isolation of a new butanol producing Clostridium strains: high level of hemicellulosic activity and structure of solventogenesis genes of a new Clostridium saccharobutylicum isolate. System. Appl. Microbiol. 32, 449-459. doi.org/10.1016/j.syapm.2009.07.005

54. Shedova, E.N., Lunina, N.A., Berezina, O.V., Zverlov, V. V., Schwarz, W. H. and Velikodworskaya, G.A. (2009). Expression of the Genes celA and xylA isolated from a fragment of metagenomic DNA in Escherichia coli. Molecular Genetics, Microbiology and Virology 24, Nr. 2, 28-32. doi.org/10.3103/S0891416809020062

53. Dvortsov, I.A., Lunina, N.A., Chekanovskaya, L.A., Schwarz, W. H., Zverlov, V. V. and Velikodworskaya, G.A. (2009). Carbohydrate binding properties of a separately folding protein module from beta-1,3-glucanase Lic16A of Clostridium thermocellum. Microbiology 155, 2442 - 2449. doi.org/10.1099/mic.0.026930-0

52. Shedova, E.N., Berezina, O.V., Lunina, N.A., Zverlov, V. V., Schwarz, W. H. and Velikodworskaya, G.A. (2009). Cloning and characterisation of a large metagenomic DNA fragment containing Glycosyl-Hydrolase genes. Molecular Genetics, Microbiology and Virology 24, Nr. 1 12-16. doi.org/10.3103/S0891416809010030

51. Shedova, E., Albrecht, C., Zverlov, V. V. and W. H. Schwarz (2009). Stimulation of bacterial DNA transformation by cattle saliva - implications for using genetically modified plants in animal feed. World Journal of Microbiology and Biotechnology 25, 457-463. doi.org/10.1007/s11274-008-9910-4

50. Zverlov, V. V. Klupp, M., Krauss, J. and Schwarz, W. H. (2008). Mutants in the scaffoldin gene cipA of Clostridium thermocellum with impaired cellulosome formation and cellulose hydrolysis: insertions of a new transposable element, IS1447, and implications for cellulase synergism on crystalline cellulose. Journal of Bacteriology 190, 4321-4327. doi.org/10.1128/JB.00097-08

49. Berezina, O.V., Sineoky, S.P., Velikodvorskaya, G.A., Schwarz, W. H., Zverlov, V. V. (2008). Extracellular Glycosyl Hydrolase Activity of the Clostridium Strains Producing Acetone, Butanol, and Ethanol. Applied Biochemistry and Microbiology 44, 42-47. doi.org/10.1134/S0003683808010079

48. Zverlov, V. V. and Schwarz, W. H. (2008). Bacterial cellulose hydrolysis in anaerobic environmental systems Clostridium thermocellum and Clostridium stercorarium, thermophilic plant fibre degraders. In "Incredible Anaerobes: From Physiology to Genomics to Fuels" Edited by Juergen Wiegel; Robert J. Maier; Michael W. W. Adams, Annals of the New York Academy of Sciences 1125, 298-307. doi.org/10.1196/annals.1419.008

47. Antoni, D., Zverlov, V. V. and Schwarz, W. H. (2007). Biofuels from Microbes. Applied Microbiology and Biotechnology 77, 23-35. doi.org/10.1007/s00253-007-1163-x

46. Berger, E., Zhang, D., Zverlov, V. V. and Schwarz, W. H. (2007). Two non-cellulosomal cellulases of Clostridium thermocellum, Cel9I and Cel48Y, hydrolyze crystalline cellulose synergistically. FEMS Microbiol. Lett 268, 194-201. doi.org/10.1111/j.1574-6968.2006.00583.x

45. Zverlov, V. V., and Schwarz, W. H. (2006). The Clostridium thermocellum cellulosome: novel components and insights from the genomic sequence. In Kataeva, I., and Ljungdahl, L. (eds.), Cellulosome. (In serial Molecular Anatomy and Physiology of Proteins, edited by Vladimir Uversky). Nova Publishers. 119-151.

44. Schwarz, W. H., and Zverlov, V. V. (2006). Protease inhibitors in bacteria: an emerging concept for the regulation of bacterial protein complexes? Molecular Microbiology 60, 1323-1326. doi.org/10.1111/j.1365-2958.2006.05181.x

43. Zverlov, V. V., Berezina, O.V., Velikodvirskaya, G.A., and Schwarz, W. H. (2006). Bacterial acetone and butanol production by industrial fermentation in the Soviet Union: use of hydrolyzed agricultural waste for biorefinery. Applied Microbiology and Biotechnology 71, 587-597. doi.org/10.1007/s00253-006-0445-z

42. Volkov, I. Yu., Lunina, N. A., Berezina, O. V., Velikodvorskaja, G. A. and Zverlov, V. V. (2005). A Thermoanaerobacter ethanolicus alfa- and beta-galactosidases gene cluster, including genes melA and lacA, and the properties of the recombinant LacA. Mol. Biology 39, 799-805. doi.org/10.1007/s11008-005-0098-5

41. Zverlov, V. V., Schantz, N., Schmitt-Kopplin, P. and Schwarz, W. H. (2005). Two new major subunits in the cellulosome of Clostridium thermocellum: xyloglucan hydrolase Xgh74A and endo-xylanase Xyn10D. Microbiology 151, 3395-3401. doi.org/10.1099/mic.0.28206-0 

40. Zverlov, V. V., Schantz, N., and Schwarz, W. H. (2005). A major new component in the cellulosome of Clostridium thermocellum is a processive endo-beta-1,4-glucanase producing cellotetraose. FEMS Microbiol. Lett 249, 353-358. doi.org/10.1016/j.femsle.2005.06.037

39. Zverlov, V. V., Kellermann, J., and Schwarz, W. H. (2005). Functional subgenomis of Clostridium thermocellum cellulosomal genes: Identification of the major components and detection of three new enzymes. Proteomics 05, 3646-3653. doi.org/10.1002/pmic.200401199

38. Zverlov, V. V., Klein M., Lucker S., Friedrich M., Kellermann J., Stahl D., Loy A., Wagner M. (2005). Lateral Gene Transfer of Dissimilatory (Bi) Sulfite Reductase Revisited. J. Bacteriol. 187, 2203-2208. doi.org/10.1128/JB.187.6.2203-2208.2005

37. Schwarz, W. H., Zverlov, V. V., Bahl, H. (2004). Extracellular glycosyl hydrolases from clostridia. In Allen I.Laskin, Joan W.Bennett, and Geoffrey M.Gadd (eds.), Adv. Appl. Microbiol. 56, 215-261. doi.org/10.1016/S0065-2164(04)56007-0

36. Zverlov, V. V., and Schwarz, W. H. (2004). The Clostridium thermocellum cellulosome - the paradigm of a multienzyme complex. In Ohmiya, K., K. Sakka, S. Karita, T. Kimura, M. Sakka, and Y. Onishi (eds.), Biotechnology of Lignocellulose Degradation and Biomass Utilization. Uni Pub. Co. Ltd., Tokyo, 137-147.

35. Adelsberger, H., Hertel, C., Glawischnig, E., Zverlov, V. V. and Schwarz, W. H. (2004). Enzyme system of Clostridium stercorarium for hydrolysis of arabinoxylan: reconstitution of the in vivo system from recombinant enzymes. Microbiology 150, 2257-2266. doi.org/10.1099/mic.0.27066-0

34. Einspanier, R., Lutz, B., Rief, S., Berezina, O., Zverlov, V., Schwarz, W., and Mayer, J. (2004). Tracing residual recombinant feed molecules during digestion and rumen bacterial diversity in cattle fed transgene maize. European Food Research and Technology 218, 269-273. doi.org/10.1007/s00217-003-0842-9

33. Veith, B., Zverlov, V. V., Lunina, N. A., Berezina, O. V., Raasch, C., Velikodvorskaya, G. A., and Liebl, W. (2003). Comparative analysis of the recombinant alfa-glucosidases from the Thermotoga neapolitana and Thermotoga maritima maltodextrin utilization gene clusters. Biocatalysis and Biotransformation 21, 147-158. doi.org/10.1080/10242420310001614324

32. Lunina N.A., Berezina O.V., Veith B., Zverlov V. V., Vorobieva I.P., Chekanovskaya L.A., Khromov I.S. , Raasch C., Liebl W., Velikodvorskaya G.A. (2003). A cluster of Thermotoga neapolitana genes involved in the degradation of starch and maltodextrins: the expression of the aglB and aglA genes in E. coli and the properties of the recombinant enzymes. Mol. Biology 37, 686-694. doi.org/10.1023/A:1026028825448

31. Berezina, O.V., Lunina, N.A., Zverlov, V. V., Naumoff, D.G., Liebl, W., Velikodvorskaya, G.A. (2003). A cluster of Thermotoga neapolitana genes involved in the degradation of starch and maltodextrins: The molecular structure of locus. Mol. Biology 37, 678-685. doi.org/10.1023/A:1026076708610

30. Fuchs, K.P., Zverlov, V. V., Velikodvorskaya, G.A., Lottspeich, F., and Schwarz, W. H. (2003). Lic16A of Clostridium thermocellum, a non-cellulosomal, highly complex endo-ß-1,3-glucanase bound to the outer cell surface. Microbiology 149, 1021-1031. https://doi.org/10.1099/mic.0.26153-0

29. Zverlov, V. V., Velikodvorskaya, G.A., Schwarz, W. H. (2003). Two new cellulosome components encoded downstream of celI in the genome of Clostridium thermocellum: the non-processive endoglucanase CelN and the possibly structural protein CseP. Microbiology 149, 515-524. https://doi.org/10.1099/mic.0.25959-0

28. Zverlov, V. V., Höll, W., Schwarz, W. H. (2003). Enzymes for digestion of cellulose and other polysaccharides in the gut of longhorn beetle larvae, Rhagium inquisitor L. (Col., Cerambycidae). International Biodeterioration and Biodegradation 51, 175-179. doi.org/10.1016/S0964-8305(02)00139-7

27. Zverlov, V. V., Fuchs, K., Schwarz, W. H. (2002). ChiA, the endochitinase in the cellulosome of the thermophilic, cellulolytic bacterium Clostridium thermocellum. Appl. Environ. Microbiology 68, 3176-3179. doi.org/10.1128/AEM.68.6.3176-3179.2002

26. Zverlov, V. V., Velikodvorskaya, G.A., Schwarz, W. H. (2002). A newly described cellulosomal cellobiohydrolase, CelO, from Clostridium thermocellum: investigation of the exo- mode of hydrolysis, and binding capacity to crystalline cellulose. Microbiology 148, 247-255. doi.org/10.1099/00221287-148-1-247

25. Zverlov, V. V., Volkov, I.Y., Velikodvorskaya, G.A., Schwarz, W. H. (2001). The binding pattern of two carbohydrate-binding modules of laminarinase Lam16A from Thermotoga neapolitana: differences in beta-glucan binding within family CBM4. Microbiology 147, 621-629. doi.org/10.1099/00221287-147-3-621

24. Zverlov, V. V., Hertel, C. , Bronnenmeier, K., Hroch, A., Kellerman, J., Schwarz, W. H. (2000). The thermostable alfa-L-rhamnosidase RamA of Clostridium stercorarium: biochemical characterization and primary structure of a bacterial alfa-L-rhamnoside hydrolase, e new type of inverting glucoside hydrolase. Mol. Microbiol. 35, 173-179. doi.org/10.1046/j.1365-2958.2000.01691.x

23. Berezina, O.V., Zverlov, V. V., Lunina, N.A., Chekanovskaya, L.A., Dubinina, E.N., Liebl, W., Velikodvorskaya, G.A. (1999). Gene and properties of thermostable 4-alfa-glucanotransferase of Thermotoga neapolitana. Mol. Biology 33, 801-806.

22. Zverlov, V. V., Schwarz, W. H. (1999). Organization of the chromosomal region containing the genes lexA and topA in Thermotoga neapolitana. Primary structure of LexA reveals phylogenetic relevance. System. Appl. Microbiol. 22, 174-178. doi.org/10.1016/S0723-2020(99)80063-0

21. Zverlov, V., Velikodvorskaya, G., Schwarz, W., Kellermann, J., Staudenbauer, W. (1999). Duplicated Clostridium thermocellum cellobiohydrolase gene encoding cellulosomal subunits S3 and S5. Appl. Microbiol. Biotechnol. 51, 852-859. doi.org/10.1007/s002530051473

20. Zverlov, V. V., Volkov, I.Yu., Lunina, N.A., Velikodvorskaya, G.A. (1999). Enzymes of thermophilic anaerobic bacteria hydrolyzing cellulose, xylan and other beta-glucans. Mol. Biology 33, 89-95.

19. Velikodvorskaya, T.V., Volkov, I.Yu., Vasilevko, V.T., Zverlov, V. V., Volkova, L.V., Alizade, Belsabane, Kh., Chekanovskaya, L.V., Piruzian, E.S. (1998). Construction and properties of a deletion variant of the laminarinase (LamA) from Thermotoga neapolitana and expression of the modified gene in protoplasts of Nocotiana plumbaginifolia. Russian J. Genetics 34, 489-492.

18. Zverlov, V. V., Liebl, W., Bachleitner, M., Schwarz, W. H. (1998). Nucleotide sequence of arfB of Clostridium stercorarium, and prediction of catalytic residues of a -L-arabinofuranosidases based on local similarity with several families of glycosyl hydrolases. FEMS Microbiol. Lett. 164, 337-343. doi.org/10.1111/j.1574-6968.1998.tb13107.x

17. Zverlov, V., Velikodvorskaya, G., Schwarz, W., Bronnenmeier, K., Kellermann, J., Staudenbauer, W. (1998). Multidomain structure and cellulosomal localization of the Clostridium thermocellum cellobiohydrolase CbhA. J. Bacteriol. 180, 3091-3099.

16. Zverlov, V., Mahr, S., Riedel, K., Bronnenmeier, K. (1998). Properties and gene structure of a bifunctional cellulolytic enzyme (celA) from the extreme thermophile Anaerocellum thermophilum with separate glycosyl hydrolase family 9 and 48 catalytic domains. Microbiol. 144, 457-465. doi.org/10.1099/00221287-144-2-457

15. Zverlov, V., Volkov, I., Velikodvorskaya, T., Schwarz, W. (1997). Thermotoga neapolitana bglB gene upstream of lamA, encodes a highly thermostable beta-glucosidase that is a laminaribiase. Microbiol. 143, 3537-3542. doi.org/10.1099/00221287-143-11-3537

14. Velikodvorskaya, T.V., Volkov, I.Yu, Vasilenko, V.T., Zverlov, V. V. and Pirusian E.S. (1997). Purification and some properties of Thermotoga neapolitana thermostable xylanase B expressed in E. coli cells. Biochemistry (Moscow) 62, 66-70.

13. Zverlov, V., Volkov, I., Velikodvorskaya, T., Schwarz, W. (1997). Higly thermostable endo-1,3-beta-glucanase (laminarinase) LamA from Thermotoga neapolitana: nucleotide sequence of the gene and characterization of the recombinant gene product. Microbiol. 143, 1701-1708. doi.org/10.1099/00221287-143-5-1701

12. Zverlov, V., Piotukh, K., Dakhova, O., Velikodvorskaya, G., Borriss, R. (1996). The multidomain xylanase A of the hyperthermophilic bacterium Thermotoga neapolitana is extremely thermoresistant. Appl. Microbiol. Biotechnol. 45, 245-247. doi.org/10.1007/s002530050678

11. Zolotarev, Yu.A., Dorochova, E.M., Nesavibatko, V.N., Borisov, Yu.A., Rosenberg, S.G., Velikodvorskaja, G.A., Neumivakin, L.V., Zverlov, V. V., Myasoedov, N.F. (1995). The solid - state catalytic synthesis of tritium - labeled amino acids, peptides and proteins. Amino Acids, 8, 353-365. doi.org/10.1007/BF00806553

10. Frolov, M.V., Zverlov, V. V., Alatortsev, V.E. (1994). The mRNA product of the Drosophila gene prune is spliced and encodes a protein containing a putative transmembrane domain. Mol. Gen. Gen., 242, 478-483. doi.org/10.1007/BF00281800

9. Zverlov, V. V., Fuchs, K.P., Schwarz, W. H., Velikodvorskaya, G.A. (1994). Purification and cellulosomal localization of Clostridium thermocellum mixed linkage ß-Glucanase LicB (1,3-1,4-b -glucanase). Biotechnol. Lett. 16, 29-34. doi.org/10.1007/BF01022619

8. Dakhova, O.N. , Kurepina, N.E., Zverlov, V. V., Svetlichnyi, V.A. and Velikodvorskaya, G.A. (1993). Cloning and expression in E. coli of Thermotoga neapolitana genes coding for Enzymes of carbohydrate substrate degradation. Biochem. Biophys. Res. Commun. 194, 1359-1364. https://doi.org/10.1006/bbrc.1993.1974

7. Tuka, K., Zverlov, V. V., Velikodvorskaja, G.A. (1992). Synergism between Clostridium thermocellum cellulases cloned in E. coli. Appl. Bochem. Biotechnol. 37/2, 201-207. doi.org/10.1007/BF02921671

6. Zverlov, V. V., Laptev, D.A., Tishkov, V.I., Velikodvorskaya, G.A. (1991). Nucleotide Sequence of the Clostridium thermocellum Laminarinase Gene. Biochem. Biophys. Res. Commun. 181, 507-512. https://doi.org/10.1016/0006-291X(91)91217-Z

5. Zverlov, V. V., Velikodvorskaya, G.A. (1990). Cloning the Clostridium thermocellum thermostable laminarinase gene in Escherichia coli; the properties of enzyme thus produced. Biotechnol. Lett. 12, 811-816. doi.org/10.1007/BF01022600

4. Tuka, K., Zverlov, V. V., Bumazkin, B.K., Velikodvorskaya, G.A., Strongin, A.Ya. (1990). Cloning and expression of Clostridium thermocellum genes coding for thermostable exoglucanases (cellobiohydrolases) in Escherichia coli cells. Biochem. Biophys. Res. Commun. 169, 1055-1060. doi.org/10.1016/0006-291X(90)92001-G

3. Zverlov, V. V., Jurjev, M.Z., Mogutov, M.A. (1990). Study of xylose conversion enzymes in yeast's. Biotekhnologiya (in Russian) 5, 21-22.

2. Velikodvorskaya, G.A., Zverlov, V. V., Laptev, D.A., Mett, B.L., Piruzian, E.S. (1990). Cloning and some properties of lih1 gene which codes for new endoglucanase of Clostridium thermocellum F7. Biotekhnologiya (in Russian) 4, 18-19.

1. Zverlov, V. V., Bankovsky, V.K., Churikova, O.V., Mogutov, M.A., Jurjev, M.Z. (1989). Cloning and expression of Zymmomonas mobilis pyruvate decarboxylase gene in E. coli. Mol. Gen., Microbiol., Virusol. (in Russian) 9, 11-13.